Macrozoobenthos is one of the biological indicators that can be used in environmental quality assessment in various aquatic ecosystems. The objective of this study was to determine the water quality in Pengujan Village based on macrozoobenthos biological indicators. This research was conducted in January-February 2021 located in Pengujan Village, Bintan Regency. Sampling was done by purposive sampling method in 5 observation stations. Macrozoobenthos was collected used a quadrant transect (1x1 m2). Parameters observed included water quality (physical and chemical parameters), as well as the type and density of macrozoobenthos. Physical and chemical parameters of the waters were compared to the value of the Quality Standards based on PP RI No. 22 of 2021, while AMBI (A Marine Biotic Index) analysis is used to determine water quality based on the presence of macrozoobenthos. The results of the measurement of water parameters are meet of the quality standard of PP RI No. 22 of 2021, except for the nitrate and phosphate parameters that do not meet the quality standards at each station. The highest density of macrozoobenthos is at station 3 which is in the dock area, while the lowest density is at station 5 which is in the barking snail catching area. Water quality based on macrozoobenthos as a water bioindicator with AMBI index all stations are included in the slightly polluted category.